Overexpression of a cytosolic NADP+-isocitrate dehydrogenase causes alterations in the vascular development of hybrid poplars.
Identifieur interne : 000D55 ( Main/Exploration ); précédent : 000D54; suivant : 000D56Overexpression of a cytosolic NADP+-isocitrate dehydrogenase causes alterations in the vascular development of hybrid poplars.
Auteurs : María Belén Pascual [Espagne] ; Juan Jesús Molina-Rueda [Espagne] ; Francisco M. Cánovas [Espagne] ; Fernando Gallardo [Espagne]Source :
- Tree physiology [ 1758-4469 ] ; 2018.
Descripteurs français
- KwdFr :
- Cytosol (enzymologie), Faisceau vasculaire des plantes (croissance et développement), Isocitrate dehydrogenases (génétique), Isocitrate dehydrogenases (métabolisme), NADP (métabolisme), Pinus (génétique), Populus (croissance et développement), Populus (enzymologie), Populus (génétique), Végétaux génétiquement modifiés (génétique).
- MESH :
- croissance et développement : Faisceau vasculaire des plantes, Populus.
- enzymologie : Cytosol, Populus.
- génétique : Isocitrate dehydrogenases, Pinus, Populus, Végétaux génétiquement modifiés.
- métabolisme : Isocitrate dehydrogenases, NADP.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Isocitrate Dehydrogenase.
- enzymology : Cytosol, Populus.
- genetics : Pinus, Plants, Genetically Modified, Populus.
- growth & development : Plant Vascular Bundle, Populus.
- chemical , metabolism : Isocitrate Dehydrogenase, NADP.
Abstract
Cytosolic NADP+-isocitrate dehydrogenase (ICDH) is one of the major enzymes involved in the production of 2-oxoglutarate for amino acid biosynthesis in plants. In most plants studied, ICDH is encoded by either one gene or a small gene family, and the protein sequence has been highly conserved during evolution, suggesting it plays different and essential roles in metabolism and differentiation. To elucidate the role of ICDH in hybrid poplar (Populus tremula x P. alba), transgenic plants overexpressing the Pinus pinaster gene were generated. Overexpression of ICDH resulted in hybrid poplar (Populus tremula × P. alba) trees with higher expression levels of the endogenous ICDH gene and higher enzyme content than control untransformed plants. Transgenic poplars also showed an increased expression of glutamine synthetase (GS1.3), glutamate decarboxylase (GAD) and other genes associated with vascular differentiation. Furthermore, these plants exhibited increased growth in height, longer internodes and enhanced vascular development in young leaves and the apical region of stem. Modifications in amino acid and organic acid content were observed in young leaves of the transgenic lines, suggesting an increased biosynthesis of amino acids for building new structures and also for transport to other sink organs, as expanding leaves or young stems. Taken together, these results support an important role of ICDH in plant growth and vascular development.
DOI: 10.1093/treephys/tpy044
PubMed: 29920606
Affiliations:
Links toward previous steps (curation, corpus...)
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Cánovas</name><affiliation wicri:level="4"><nlm:affiliation>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Campus Universitario de Teatinos, Málaga, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Campus Universitario de Teatinos, Málaga</wicri:regionArea><orgName type="university">Université de Malaga</orgName><placeName><settlement type="city">Malaga</settlement><region nuts="2" type="region">Andalousie</region></placeName></affiliation></author><author><name sortKey="Gallardo, Fernando" sort="Gallardo, Fernando" uniqKey="Gallardo F" first="Fernando" last="Gallardo">Fernando Gallardo</name><affiliation wicri:level="4"><nlm:affiliation>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Campus Universitario de Teatinos, Málaga, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Campus Universitario de Teatinos, Málaga</wicri:regionArea><orgName type="university">Université de Malaga</orgName><placeName><settlement type="city">Malaga</settlement><region nuts="2" type="region">Andalousie</region></placeName></affiliation></author></analytic><series><title level="j">Tree physiology</title><idno type="eISSN">1758-4469</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cytosol (enzymology)</term><term>Isocitrate Dehydrogenase (genetics)</term><term>Isocitrate Dehydrogenase (metabolism)</term><term>NADP (metabolism)</term><term>Pinus (genetics)</term><term>Plant Vascular Bundle (growth & development)</term><term>Plants, Genetically Modified (genetics)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cytosol (enzymologie)</term><term>Faisceau vasculaire des plantes (croissance et développement)</term><term>Isocitrate dehydrogenases (génétique)</term><term>Isocitrate dehydrogenases (métabolisme)</term><term>NADP (métabolisme)</term><term>Pinus (génétique)</term><term>Populus (croissance et développement)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Végétaux génétiquement modifiés (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Isocitrate Dehydrogenase</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Faisceau vasculaire des plantes</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Cytosol</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Cytosol</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Pinus</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Vascular Bundle</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Isocitrate dehydrogenases</term><term>Pinus</term><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Isocitrate Dehydrogenase</term><term>NADP</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Isocitrate dehydrogenases</term><term>NADP</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cytosolic NADP+-isocitrate dehydrogenase (ICDH) is one of the major enzymes involved in the production of 2-oxoglutarate for amino acid biosynthesis in plants. In most plants studied, ICDH is encoded by either one gene or a small gene family, and the protein sequence has been highly conserved during evolution, suggesting it plays different and essential roles in metabolism and differentiation. To elucidate the role of ICDH in hybrid poplar (Populus tremula x P. alba), transgenic plants overexpressing the Pinus pinaster gene were generated. Overexpression of ICDH resulted in hybrid poplar (Populus tremula × P. alba) trees with higher expression levels of the endogenous ICDH gene and higher enzyme content than control untransformed plants. Transgenic poplars also showed an increased expression of glutamine synthetase (GS1.3), glutamate decarboxylase (GAD) and other genes associated with vascular differentiation. Furthermore, these plants exhibited increased growth in height, longer internodes and enhanced vascular development in young leaves and the apical region of stem. Modifications in amino acid and organic acid content were observed in young leaves of the transgenic lines, suggesting an increased biosynthesis of amino acids for building new structures and also for transport to other sink organs, as expanding leaves or young stems. Taken together, these results support an important role of ICDH in plant growth and vascular development.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29920606</PMID><DateCompleted><Year>2018</Year><Month>12</Month><Day>27</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>27</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1758-4469</ISSN><JournalIssue CitedMedium="Internet"><Volume>38</Volume><Issue>7</Issue><PubDate><Year>2018</Year><Month>07</Month><Day>01</Day></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Overexpression of a cytosolic NADP+-isocitrate dehydrogenase causes alterations in the vascular development of hybrid poplars.</ArticleTitle><Pagination><MedlinePgn>992-1005</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tpy044</ELocationID><Abstract><AbstractText>Cytosolic NADP+-isocitrate dehydrogenase (ICDH) is one of the major enzymes involved in the production of 2-oxoglutarate for amino acid biosynthesis in plants. In most plants studied, ICDH is encoded by either one gene or a small gene family, and the protein sequence has been highly conserved during evolution, suggesting it plays different and essential roles in metabolism and differentiation. To elucidate the role of ICDH in hybrid poplar (Populus tremula x P. alba), transgenic plants overexpressing the Pinus pinaster gene were generated. Overexpression of ICDH resulted in hybrid poplar (Populus tremula × P. alba) trees with higher expression levels of the endogenous ICDH gene and higher enzyme content than control untransformed plants. Transgenic poplars also showed an increased expression of glutamine synthetase (GS1.3), glutamate decarboxylase (GAD) and other genes associated with vascular differentiation. Furthermore, these plants exhibited increased growth in height, longer internodes and enhanced vascular development in young leaves and the apical region of stem. Modifications in amino acid and organic acid content were observed in young leaves of the transgenic lines, suggesting an increased biosynthesis of amino acids for building new structures and also for transport to other sink organs, as expanding leaves or young stems. Taken together, these results support an important role of ICDH in plant growth and vascular development.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pascual</LastName><ForeName>María Belén</ForeName><Initials>MB</Initials><AffiliationInfo><Affiliation>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Campus Universitario de Teatinos, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Molina-Rueda</LastName><ForeName>Juan Jesús</ForeName><Initials>JJ</Initials><AffiliationInfo><Affiliation>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Campus Universitario de Teatinos, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cánovas</LastName><ForeName>Francisco M</ForeName><Initials>FM</Initials><AffiliationInfo><Affiliation>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Campus Universitario de Teatinos, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gallardo</LastName><ForeName>Fernando</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Campus Universitario de Teatinos, Málaga, Spain.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>53-59-8</RegistryNumber><NameOfSubstance UI="D009249">NADP</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.1.41</RegistryNumber><NameOfSubstance UI="D007521">Isocitrate Dehydrogenase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.1.42</RegistryNumber><NameOfSubstance UI="C039355">isocitrate dehydrogenase (NADP+)</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D003600" MajorTopicYN="N">Cytosol</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007521" MajorTopicYN="N">Isocitrate Dehydrogenase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009249" MajorTopicYN="N">NADP</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D028223" MajorTopicYN="N">Pinus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058526" MajorTopicYN="N">Plant Vascular Bundle</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>11</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>04</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>6</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>12</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>6</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29920606</ArticleId><ArticleId IdType="pii">5038980</ArticleId><ArticleId IdType="doi">10.1093/treephys/tpy044</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li></country><region><li>Andalousie</li></region><settlement><li>Malaga</li></settlement><orgName><li>Université de Malaga</li></orgName></list><tree><country name="Espagne"><region name="Andalousie"><name sortKey="Pascual, Maria Belen" sort="Pascual, Maria Belen" uniqKey="Pascual M" first="María Belén" last="Pascual">María Belén Pascual</name></region><name sortKey="Canovas, Francisco M" sort="Canovas, Francisco M" uniqKey="Canovas F" first="Francisco M" last="Cánovas">Francisco M. Cánovas</name><name sortKey="Gallardo, Fernando" sort="Gallardo, Fernando" uniqKey="Gallardo F" first="Fernando" last="Gallardo">Fernando Gallardo</name><name sortKey="Molina Rueda, Juan Jesus" sort="Molina Rueda, Juan Jesus" uniqKey="Molina Rueda J" first="Juan Jesús" last="Molina-Rueda">Juan Jesús Molina-Rueda</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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